陈士林

中文名陈士林

出生地中国

代表作品中药饮片标准汤剂、经典名方开发指引、中国药典中药材DNA条形码标准序列、本草基因组学

民族汉

职务首席教授

目录导航

科研成就

陈士林创建了基于ITS2的中草药DNA条形码鉴定方法体系，完成专著《中国药典中药材DNA条形码标准序列》，从基因层面解决中草药物种真伪鉴定的难题，被评为2016中国十大医学进展^[19]；

通过全基因组解析提出灵芝为首个中药基原药用模式真菌，被Nature China 选为中国最佳研究亮点推介^[20]；

完成并发表人参^[21]、丹参^[22]、赤芝^[23]、菊花^[24]、卷柏^[25]、穿心莲^[26]、紫芝^[27]、紫苏^[28]、黄连^[29]、红豆杉^[30]、黄花蒿^[31]等全基因组图谱和相关组学研究，成功培育并获批11个中药材新品种证书或良种证书，提出并主编《本草基因组学》学科由科学出版社出版，列为全国高等医药院校规划教材，出版《药用植物分子遗传学》，奠定药用植物分子遗传学基础^[32]。

完成并编著《中国中药材产地生态适宜性数值区划》，避免中药材盲目引种栽培^[33]；

主要论文

1. Sun, S., Shen, X., Li, Y., Li, Y., Wang, S., Li, R., Zhang, H., Shen, G., Guo, B., Wei, J., Xu, J., St-Pierre, B., Chen, S., & Sun, C. Single-cell RNA sequencing provides a high-resolution roadmap for understanding the multicellular compartmentation of specialized metabolism. Nature plants. 2023. 9(1), 179–190.

2. Chen, S., Xu, J., Liu, C., Zhu, Y., Nelson, D. R., Zhou, S., Li, C., Wang, L., Guo, X., Sun, Y., Luo, H., Li, Y., Song, J., Henrissat, B., Levasseur, A., Qian, J., Li, J., Luo, X., Shi, L., He, L., … Sun, C. Genome sequence of the model medicinal mushroomGanoderma lucidum.Nature Communications. 2012, 3, 913.

3. Yujun, Zhang., Qi, Shen.,Liang, Leng.,Dong, Zhang.,Sha, Chen.,Yuhua, Shi.,Zemin, Ning.,& Shilin, Chen. Incipient diploidization of the medicinal plantPerillawithin 10,000 years，Nature Communications. 2021, 12(1):5508.

4. Liu Y, Wang B, Shu S, Li Z, Song C, Liu D, Niu Y, Liu J, Zhang J, Liu H, Hu Z, Huang B, Liu X, Liu W, Jiang L, Alami MM, Zhou Y, Ma Y, He X, Yang Y, Zhang T, Hu H, Barker MS, Chen S, Wang X, Nie J. Analysis of the Coptis chinensis genome reveals the diversification of protoberberine-type alkaloids. Nature Communications. 2021, 12(1):3276.

5. Chen S., Song, J., Sun, C., Xu, J., Zhu, Y., Verpoorte, R., Fan, T.-P*. Herbal genomics: Examining the biology of traditional medicines. Science.2015,347(6219), S27-S29.

6. China Plant BOL Group, Li DZ, Gao LM, Li HT, Wang H, Ge XJ, Liu JQ, Chen ZD, Zhou SL, Chen SL, Yang JB, Fu CX, Zeng CX, Yan HF, Zhu YJ, Sun YS, Chen SY, Zhao L, Wang K, Yang T, Duan GW. Comparative analysis of a large dataset indicates that internal transcribed spacer (ITS) should be incorporated into the core barcode for seed plants. PNAS. 2011,108:19641-19646.

7. Liao B, Shen X, Xiang L, Guo S, Chen S, Meng Y, Liang Y, Ding D, Bai J, Zhang D, Czechowski T, Li Y, Yao H, Ma T, Howard C, Sun C, Liu H, Liu J, Pei J, Gao J, Wang J, Qiu X, Huang Z, Li H, Yuan L, Wei J, Graham I, Xu J, Zhang B, Chen S*. Allele-aware chromosome-level genome assembly of Artemisia annua reveals the correlation between ADS expansion and artemisinin yield. Molecular plant. 2022, 5(8):1310-1328.

8. Song, C; Liu, Y; Song, A; Dong, G; Zhao, H; Sun, W; Ramakrishnan, Shyam; Wang, Y; Wang, S; Li, T; Niu, Y; Jiang, J; Dong, B; Xia, Y; Chen, S; Hu, Z; Chen, F; Chen, Shilin, The Chrysanthemum nankingense genome provides insights into the evolution and diversification of chrysanthemum flowers and medicinal traits.Molecular plant. 2018, 11(12): 1482-1491.

9. Chen, S., Yin, X., Han, J., Sun, W., Yao, H., Song, J., & Li, X. DNA barcoding in herbal medicine: retrospective and prospective. Journal of Pharmaceutical Analysis. 2023.

10. Chen, X., Yang, Z., Xu, Y., Liu, Z., Liu, Y., Dai, Y., & Chen, S. Progress and prediction in multicomponent quantification of complex systems with practical LC-UV methods. Journal of Pharmaceutical Analysis, 2022.

11. Guo M, Pang X, Xu Y, Jiang W, Liao B, Yu J, Xu J, Song J, Chen S. Plastid genome data provide new insights into the phylogeny and evolution of the genus Epimedium. Journal of Advanced Research. 2021, 36:175-185.

12. Xu J, Chu Y, Liao B, Xiao S, Yin Q, Bai R, Su H, Dong L, Li X, Qian J, Zhang J, Zhang Y, Zhang X, Wu M, Zhang J, Li G, Zhang L, Chang Z, Zhang Y, Jia Z, Liu Z, Daniel Afreh, Ruth Nahurira8, Zhang L, Cheng R, Zhu Y, Zhu G, Rao W, Zhou C, Qiao L, Huang Z, Cheng Yung-Chi, Chen Shilin, Panax ginseng genome examination for ginsenoside biosynthesis.GigaScience. 2017, 6(11):1-15.

13. Chen, S., Li, Z., Zhang, S., Zhou, Y., Xiao, X., Cui, P., Xu, B., Zhao, Q., Kong, S., & Dai, Y. (2022). Emerging biotechnology applications in natural product and synthetic pharmaceutical analyses. Acta Pharmaceutica Sinica B, 12(11).

14. Xu J, Guo S, Yin X, Li M, Su H, Liao X, Li Q, Le L, Chen S, Liao B, Hu H, Lei J, Zhu Y, Qiu X, Luo L, Chen J, Cheng R, Chang Z, Zhang H, Wu NC, Guo Y, Hou D, Pei J, Gao J, Hua Y, Huang Z, Chen S*. Genomic, transcriptomic, and epigenomic analysis of a medicinal snake, Bungarus multicinctus, to provides insights into the origin of Elapidae neurotoxins, Acta Pharmaceutica Sinica B. https://doi.org/10.1016/j.apsb.2022.11.015.

15. Luo L, Jiang J, Wang C, Fitzgerald M, Hu W, Zhou Y, Zhang H, Chen S. Analysis on herbal medicines utilized for treatment of COVID-19.Acta Pharmaceutica Sinica B.2020, 10: 1192-1204.

16. Luo L, Dong L, Huang Q, Ma S, Fantke P, Li J, Jiang J, Fitzgerald M, Yang J, Jia Z, Zhang J, Wang H, Dai Y, Zhu G, Xing Z, Liang Y, Li M, Wei G, Song J, Wei J, Peng C, Zhang H, Zhang W, Wang S, Mizuno K, Marco A, Wu L, Xu J, Xiong C, Chen S. Detection and risk assessments of multi-pesticides in 1771 cultivated herbal medicines by GC/MS-MS and LC/MS-MS. Chemosphere. 2021,262:127477.

17. Z Xu, T Xin, Dorothea Bartels, Y Li, W Gu, H Yao, S Liu, H Yu, X Pu, J Zhou, J Xu, C Xi, H Lei Jing, yuan Song, Shilin Chen. Genome analysis of the ancient tracheophyte Selaginella tamariscinareveals evolutionary features relevant to the acquisition of desiccation tolerance. Molecular plant. 2018.

18. Shilin Chen.; Pang, X.; Song, J.; Shi, L.; Yao, H.; Han, J.; Leon, C., A renaissance in herbal medicine identification: from morphology to DNA. Biotechnology advances. 2014, 32 (7), 1237-1244.

19. H Xu, J Song, H Luo, Y Zhang, Q Li, Y Zhu, J Xu, Y Li, C Song, B Wang, W Sun, G Shen, X Zhang, J Qian, A Ji, Z Xu, X Luo, L He, C Li, C Sun, H Yan, G Cui, X Li, Xi Li, J Wei, J Liu,Y Wang, A Hayward, D Nelson, Z Ning, RJ. Peters, X Qi, S Chen. Analysis of the Genome Sequence of the Medicinal PlantSalvia miltiorrhiza.Molecular plant. 2016, 9(6): p949–952.

20. Zheng X, An W, Yao H, Xu J, Chen S. Rapid authentification of the poisonous plantGelsemium elegansby combining Filter-Paper-Based DNA extraction and RPA-LFD detection.Engineering.2021,7(1):14-16.

21. Li, X.; Yang, Y.; Henry, R. J.; Rossetto, M.; Wang, Y.; ShiLin Chen, Plant DNA barcoding: from gene to genome.Biological Reviews.2015, 90 (1), 157-166.

22. Li, Q.; Li, Y.; Song, J.*; Xu, H.; Xu, J.; Zhu, Y.; Li, X.; Gao, H.; Dong, L.; Qian, J.; Sun, C, Shilin Chen., High-accuracy de novo assembly and SNP detection of chloroplast genomes using a SMRT circular consensus sequencing strategy.New Phytologist. 2014, 204 (4), 1041-1049.