Western blotting
提取蛋白质样品进行电泳分离,然后转移至薄膜,然后用抗体通过免疫学反应检测目的蛋白,分析基因的表达程度。
involves the transfer of electrophoretically separated protein molecules to membranes. The membrane is then probed with an antibody to detect the protein of interest, in a similar way to immunological screening of plaque lifts from expression libraries.
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